A.

A.

以下是來自廠商的訊息：

Antibodies used to detect GFP on western-We tried to use several commercial antibodies against GFP, and found that most did not work well at all. The best result we had was with Roche's mixed monoclonal antibodies (Cat #1814460) used at a dilution of 1:500 or less. Eventually we got some anti-sera from a colleague on the East coast that had made some for his own work, and this seemed to work a bit better (i.e., at 1:5000). Invitrogen Anti-GFP and Sana Cruz Anti GFP DO NOT work.

In summary, invitrogen’s R&D has also verified the clones by western blotting and they recommend to use Roche's GFP antibody. Please consider trying that and it should give the customer some signal of his clone.