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雷射與集光濾鏡組態
分析儀1-3號機 FACSCalibur
Excitation Laser (nm) |
Fluorescence Channel |
Bandpass Filter (nm) |
Color | Fluorophores and other useful dyes | |
---|---|---|---|---|---|
488 | FL1 | 530/30 | Green | Alexa Fluor 488, FITC, GFP, |
Fluo-3 or Fluo-4, Calcein, CFSE, GFP, Rhodamine123, TO-PRO®-1, YO-PRO®-1, Click-i EdU Alexa Fluor 488, JC-1 or DiOC2(3), SYTOX® Green, DyeCycle™ Green LIVE/DEAD® Fixable Green Dead Cell Stain |
FL2 | 585/42 | Yellow/ Orange |
PE, DsRed, |
PI, Fura Red, DyeCycle™ Orange, JC-1 or DiOC2(3), SNARF® (low pH), pHrodo™ dye |
|
FL3 | 670LP | Dark Red | PE-Cy5.5, PE-Cy7 PerCP, PerCP-Cy5.5, PE-Alexa Fluor 700, |
PI, 7-AAD, SNARF® (high pH), DiOC2(3), LIVE/DEAD® Fixable Red Dead Cell Stain |
|
635 | FL4 | 661/16 | Red | APC, Alexa Fluor 647, |
Click-i EdU Alexa Fluor 647, LIVE/DEAD® Fixable Far Red Dead Cell Stain, TO-PRO®-3, SYTOX® Red, MitoProbe™ DiIC1(5), CellTrace Far Red DDAO-SE |
** 若需要,本表所列出的螢光試劑當然亦可適用於其他具有類似雷射/集光濾 鏡組態的流式細胞儀。