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儀器設備儀器設備
雷射與集光濾鏡組態
分選儀1號機 FACSAriaIIIu
| Excitation Laser (nm) |
Dichroic mirror | Longpass Filter (nm) |
Bandpass Filter (nm) |
Fluorophores and other useful dyes |
|---|---|---|---|---|
| 488 | A | 655 LP | 695/40 | PerCP (87.4/63.3), PerCP-Cy5.5 (98.4/68.1), PE-Cy5.5 (76.9/38.5) |
| B | 502 LP | 530/30 | Alexa Fluor 488, FITC, GFP, Fluo-3 or Fluo-4 | |
| C | – | 488/10 | SSC | |
| 561 | A | 735 LP | 780/60 | PE-Cy7 (94.9/60.5) |
| B | 630 LP | 670/14 | PE-Cy5 (91.9/29.2), 7-AAD | |
| C | 600 LP | 610/20 | PE-Texas Red (94.6/30), PI DsRed (85.8/20.1) mCherry, |
|
| D | – | 582/15 | dTomato, PE(94.3/~30), RFP, Cy3 DsRed (85.8/22.3), |
|
| 633 | A | 735 LP | 780/60 | APC-Cy7, APC-H7, Alexa Fluor® 750 |
| B | – | 660/20 | APC, Alexa Fluor® 647, Cy5, MitoProbe™ DiIC1(5), CellTrace™ Far Red DDAO-SE, SYTOX® Red |
|
| 405 | A | 630 LP | 660/20 | Brilliant Violet650, Qdot 655 |
| B | 600 LP | 610/20 | Brilliant Violet605, Qdot 605 | |
| C | 556 LP | 585/42 | Brilliant Violet570, Qdot565 | |
| D | 502 LP | 510/50 | Brilliant Violet510, AmCyan, Horizon V500, | |
| E | – | 450/40 | Pacific Blue, Brilliant Violet421, Alexa Fluor® 405, Horizon V450, |
** 加註刪除線者表示儀器組態中有2個或2個以上的位置可以收集到該螢光訊號，加註刪除線 的位置非該螢光試劑之最佳收集位置，建議以未加註刪除線之位置收集其螢光訊號。
