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雷射與集光濾鏡組態
分選儀2號機FACSAria II
| Excitation Laser (nm) |
Dichroic mirror | Longpass Filter (nm) |
Bandpass Filter (nm) |
Fluorophores |
|---|---|---|---|---|
| 488 | A | 735 LP | 780/60 | PE-Cy7 (61.8/60.5) |
| B | 655 LP | 695/40 | PerCP (87.4/63.3), PerCP-Cy5.5 (98.4/68.1), PE-Cy5.5 (76.9/38.5), |
|
| C | 610 LP | 616/23 | PE-Texas Red (94.6/35), PE-Alexa Fluor® 610 |
|
| D | 556 LP | 585/42 | DsRed (40/59.7), PE (61.6/70.4), Cy3 |
|
| E | 502 LP | 530/30 | Alexa Fluor 488, FITC, GFP, Fluo-3 or Fluo-4, Calcein, TO-PRO®-1, YO-PRO®-1, CFSE, Rhodamine123… | |
| F | – | 488/10 | SSC | |
| 561 | A | 735LP | 780/60 | PE-Cy7 |
| B | 630LP | 670/14 | PE-Cy5.5 | |
| C | 600LP | 610/20 |
PE-Texas Red, mCherry DsRed (85.8/20.1), |
|
| D | - | 582/15 |
dTomato, RFP, Cy3, PE DsRed (85.8/22.3) |
|
| 633 | A | 735 LP | 780/60 | APC-Cy7, APC-H7, Alexa Fluor® 750 |
| B | - | 660/20 | APC, Alexa Fluor® 647, Cy5 | |
| 375 (NUV) | A | 610LP | 670LP | Hoechst Red |
| B | – | 450/40 | Hoechst Blue33258 (57.9/38.3), DAPI (79.8/36.9) |
|
| – | 502LP | 530/30 | 未使用 | |
| – | - | 450/20 | 未使用 |
