Please note that any expression library will have 2/3 of the library out of frame (66%). However, even when clones are out of frames, yeast are able to tolerate translational frameshifts. The sequence provided shows some open reading. The customer can be re-cloned in frame and proceed to screen again make sure all 4 reporters are active.
國立台灣大學醫學院第一共同研究室生醫資源中心 (基醫大樓R1439/R1438)
Biomedical Resource Core of the First Core Laboratory, College of Medicine, National Taiwan University