儀器設備
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儀器設備
樣品分析-生物膜干涉感測器 (建置中)
Octet R8 (Sartorius)
@規格
| Number of spectrometers | 8 |
| Maximum simultaneous reads | 8 |
| Data collection rate | 2, 5 or 10 Hz |
| Sample position and format | 1 standard 96-well, black, flat bottom microplate |
| Sample volume | 180–220 μL/well, non-destructive testing |
| Orbital flow capacity | Static or 100–1500 rpm |
| Analysis temperature range | 15–40°C, 1°C increments |
@功能
蛋白質-蛋白質/蛋白質-胜肽交互作用力分析
蛋白質-核酸交互作用力分析
蛋白質-脂質交互作用力分析
蛋白質-小分子交互作用力分析
抗體與抗體片段表徵分析
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輝光放電機
easiGlow (PELCO)
@規格
| Plasma current | 0 – 30 mA |
| Working vacuum | 0.20 – 1.1 mbar for optimal glow |
| Negative and positive polarity |
@功能
改變網格表面性質
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冷凍樣品製備機 (開放線上預約)
Vitrobot IV (TFS)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Double side |
@功能
冷凍網格製備
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冷凍樣品製備機 II (開放線上預約)
GP2 (Leica)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Single side |
@功能
冷凍網格製備
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冷凍電子顯微鏡 (需與儀管人員協同操作)
Glacios Cryo-TEM (TFS)
@規格
| Accelerating Voltage | 200 kV |
| Electron Source | High-brightness field emission gun (X-FEG) |
| Stage | Computerized 4-axes specimen stage with ±70° alpha tilt (Compustage) |
| Objective Apertures | 100, 70 and Volta Phase Plate |
| Spherical Aberration | 2.7 mm |
| Cryo-holder | Automated loading of grids from cassettes, containing up to 12 Autogrids |
| Imaging Software | EPU 3.8.1 & Tomography 5.21 |
| Detectors |
Ceta-D (4096x4096, 14 μm2 pixel CMOS) Falcon4 (4096x4096, 14 μm2 Direct Electron Detection) |
| Image pixel size (Å) | 1.6 @92 kx ~ 0.74 @190 kx w/ Falcon 4 |
@功能
單粒子分析 (Single Particle Analysis, SPA) (蛋白質複合體、病毒結構、脂質體...)
冷凍斷層掃瞄 (Cryo-Tomography, CryoET) (病毒結構、細胞及切片樣品)
微晶體繞射 (Microcrystal Electron Diffraction, MicroED)
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高壓冷凍儀 (使用請洽劉淦光博士)
EM ICE (Leica)
@規格
|
Time between freezing cycles |
1 minute |
| Freeze specimen with 2000 atm pressure within milliseconds | |
| Programmable rotation system enabling 9 freezing cycles |
@功能
EM ICE 使用高壓冷凍固定生物樣品以保存其自然狀態下的精細結構。此儀器配備有自動樣品加載系統，以利將樣品迅速轉移到液氮中完成冷凍固定，從而保證過程的可重複性。高壓冷凍固定可在不引起顯著結構改變情況下固定細胞。
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樣品冷凍置換工作站 (使用請洽劉淦光博士)
EM AFS2 (Leica)
@規格
| Dewar capacity for liquid nitrogen | 35 liters |
| Liquid nitrogen consumption during cooling | 5 liters |
| UV LED range | 374-383 nm |
@功能
EM AFS2 是一台進行樣品冷凍置換的儀器，主要用於冷凍替代和梯度變溫技術，同時也可以用於樹脂的低溫包埋和聚合過程。
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