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2016/03/15test
| Excitation Laser (nm) |
Dichroic mirror | Longpass Filter (nm) |
Bandpass Filter (nm) |
Fluorophores & other useful dyes |
|---|---|---|---|---|
| 355 (UV) |
A | 505LP(暫) | 530/30(暫) | Hoechst Red, BUV803, BUV737, BUV661, BUV496 |
| B | 450/50(暫) | Hoechst Blue, BUV395 | ||
| 替 換 用 濾 鏡 |
770LP | 820/60 | BUV803 | |
| 690LP | 740/35 | BUV737 | ||
| 610LP | 670LP | Hoechst Red | ||
| 630LP | 670/25 | BUV661 | ||
| 450LP | 515/30 | BUV496 | ||
| 450/50 | Hoechst Blue | |||
| 379/28 | BUV395 | |||
| 405 (Violet) |
A | 685LP | 710/50 | BV711 |
| B | 630LP | 655/8 | BV650, Qdot 655 | |
| C | 595LP | 605/12 | BV605, Qdot 605 | |
| D | 545LP | 560/20 | BV570, Qdot565 | |
| E | 475LP | 525/50 | BV510, AmCyan, Qdot525, Horizon V500, SYTOX Blue, Indo-1 (low Ca2+) |
|
| F | - | 450/50 | BV421, Pacific Blue, eFluor 450, Horizon V450, Alexa Fluor 405, DyeCycle Violet |
|
| 488 (Blue) |
A | 685LP | 695/40 | PerCP , PerCP-Cy5.5 |
| B | 505LP | 530/30 | Alexa Fluor 488, FITC, GFP, CFSE, YO-PRO®-1, JC-1 Fluo-3或Fluo-4, Calcein, TO-PRO®-1, Rhodamine123… |
|
| C | - | 488/10 | SSC | |
| 561 (yellow green) |
A | 750LP | 780/60 | PE-Cy7 |
| B | 685LP | 710/50 | PE-Cy5.5 7-AAD-B, PI-B |
|
| C | 635LP | 670/30 | PE-Cy5 7-AAD-C, PI-C |
|
| D | 600LP | 610/20 | PE-Texas Red, mCherry, PI |
|
| E | 582/15 | PE, dTomato, DsRed, RFP, Cy3 | ||
| 640 (Red) |
A | 750LP | 780/60 | APC-Cy7, APC-H7, Alexa Fluor® 750 |
| B | 710LP | 730/45 | Alexa Fluor 700, Cy5.5 | |
| C | - | 670/14 | APC, Aexa Fluor® 647, Cy5, MitoProbe™ DiIC1(5), SYTOX Red |
