儀器設備
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儀器設備
冷凍樣品製備機 I
Vitrobot IV (TFS)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Single side |
@服務內容
冷凍樣品製備
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冷凍樣品製備機 II
GP2 (Leica)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Single side |
@服務內容
冷凍樣品製備
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冷凍電子顯微鏡
Glacios Cryo-EM (TFS)
@規格
| Accelerating voltage | 200 kV |
| Electron source | High-brightness field emission gun (X-FEG) |
| Stage | Computerized 4-axes specimen stage with ±70° alpha tilt (Compustage) |
| Objective Apertures | 100, 70 and Volta Phase Plate |
| Spherical Aberration (Cs) | 2.7 mm |
| Cryo-holder | Automated loading of grids from cassettes, containing up to 12 Autogrids |
| Automated software available | EPU and Tomography |
| Camera | Ceta-D & Falcon4i (14×14 μm2 pixel size, 4096×4096 pixels) |
| Pixel size (Å) | 0.934 @150 kx; 0.574 @240 kx with Falcon 4i |
@服務內容
1.單粒子分析 (Single Particle Analysis, SPA) (蛋白質複合體、病毒結構、脂質體...)
2.冷凍斷層掃瞄 (Cryo-Tomography, CryoET) (病毒結構、細胞及切片樣品)
3.微晶體繞射 (Microcrystal Electron Diffraction, MicroED, 技術建置中…)
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高壓冷凍樣品製備機
EM ICE (Leica)
@規格
| Freeze specimen with 2000 atm pressure within milliseconds | |
| Programmable rotation system enabling 9 freezing cycles | |
| Time between freezing cycles | 1 minute |
@服務內容
冷凍樣品製備
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樣品冷凍置換機
EM AFS2 (Leica)
@規格
| Dewar capacity for liquid nitrogen | 35 liters |
| Liquid nitrogen consumption during cooling | 5 liters |
| UV LED range | 374-383 nm |
@服務內容
樣品冷凍置換
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