儀器設備
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儀器設備
冷凍樣品製備機 (開放線上預約)
Vitrobot IV (TFS)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Double side |
@服務內容
冷凍網格製備
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冷凍電子顯微鏡 (開放線上預約)
Glacios Cryo-TEM (TFS)
@規格
| Accelerating voltage | 200 kV |
| Electron source | High-brightness field emission gun (X-FEG) |
| Stage | Computerized 4-axes specimen stage with ±70° alpha tilt (Compustage) |
| Objective Apertures | 100, 70 and Volta Phase Plate |
| Spherical Aberration (Cs) | 2.7 mm |
| Cryo-holder | Automated loading of grids from cassettes, containing up to 12 Autogrids |
| Automated software available | EPU 3.8 and Tomography 5.16 |
| Detectors |
Ceta-D & Falcon4 (Sensor size: 4096×4096 pixels, Pixel size: 14×14 μm2 ) |
| Image pixel size (Å) | 1.6 @92 kx ~ 0.74 @190 kx with Falcon 4i |
@服務內容
1.單粒子分析 (Single Particle Analysis, SPA) (蛋白質複合體、病毒結構、脂質體...)
2.冷凍斷層掃瞄 (Cryo-Tomography, CryoET) (病毒結構、細胞及切片樣品)
3.微晶體繞射 (Microcrystal Electron Diffraction, MicroED)
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冷凍樣品製備機 II (預約使用請與劉淦光博士聯繫)
GP2 (Leica)
@規格
| Working Temperature | 4 – 60 °C |
| Humidity Range | Maintain relative humidity at 100% |
| Blotting Control | Single side |
@服務內容
冷凍網格製備
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高壓冷凍儀 (預約使用請與劉淦光博士聯繫)
EM ICE (Leica)
@規格
|
Time between freezing cycles |
1 minute |
| Freeze specimen with 2000 atm pressure within milliseconds | |
| Programmable rotation system enabling 9 freezing cycles |
@儀器簡介
EM ICE 使用高壓冷凍固定生物樣品以保存其自然狀態下的精細結構。此儀器配備有自動樣品加載系統，以利將樣品迅速轉移到液氮中完成冷凍固定，從而保證過程的可重複性。高壓冷凍固定可在不引起顯著結構改變情況下固定細胞。
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樣品冷凍置換工作站 (預約使用請與劉淦光博士聯繫)
EM AFS2 (Leica)
@規格
| Dewar capacity for liquid nitrogen | 35 liters |
| Liquid nitrogen consumption during cooling | 5 liters |
| UV LED range | 374-383 nm |
@儀器簡介
EM AFS2 是一台進行樣品冷凍置換的儀器，使用漸進降溫技術，可接續進行低溫樹脂包埋和聚合。
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